Vaccine antigen archiving by lymphatic endothelial cells boosts archived antigen-specific CD8 T cell memory responses during a pathogenic challenge

Abstract Numerous studies have shown viral antigens can persist in lymph nodes after the resolution of infection. We demonstrated that lymphatic endothelial cells (LEC), which comprise vasculature necessary for antigen drainage from tissue, are predominant cell type required persistence both vaccine and within node. termed this process archiving due to ability LEC actively archive an immune response has occurred. This involves acquisition, retention, exchange between LECs dendritic (DC), resulting presentation archived antigen-specific CD8 memory T cells. In study, we evaluated effect on during a pathogenic challenge. predicted challenge would result release subsequent presentation. Following challenge, found significant increase endogenous or transferred cells, compared bystander activation seen by unrelated specificity. The phenotype was more effector-like led protection against Listeria monocytogenes (LM)-expressing antigen, local area. conclusion, these findings suggest may be released As such, acquired migratory DCs as they traffic through lymphatics order present acting effective boost. NIH R01 AI121209